Analysis Note

ControlPancreas, adrenal gland, bowel and thyroid tissues Immunoblot: PC12 cell lysate, human pancreatic tissue

Application

Immunohistochemistry:
A 1-10 µg/mL concentration of a previous lot was used in IH.

Optimal working dilutions must be determined by end user.

Detect Chromogranin A using this Anti-Chromogranin A Antibody, clone LK2H10 validated for use in IH, IH(P) & WB.

General description

Chromogranin A (a protein of 439 amino acids which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: CBL219

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Packaging

500 µg in 500 μL

500 µL

Physical form

Purified mouse monoclonal IgG1 in buffer containing 0.05M Borate buffered saline, 0.15M NaCl, pH 8.2 with 0.1% sodium azide.

Format: Purified

Quality

Routinely evaluated by Western Blot on Mouse Pancreas lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected Chromogranin A on 10 µg of Mouse Pancreas lysate.

Specificity

Chromogranin, an acidic protein having a molecular weight of about 68 kDa abides in the polypeptide-hormone-containing secretory granules of human endocrine tissues. Anti-chromogranin A can be used to stain most normal and neoplastic endocrine tissues containing secretory granules (exceptions include cells from the hypothalamus and posterior pituitary, which are known to contain secretory granules). The amount of staining is usually proportional to the number of secretory granules present (1).

Target description

50 kDa